Comparison of different poultry egg yolks-citrate extender with green tea (Camellia sinensis) extract addition on Sapudi ram spermatozoa quality in chilled temperature storage

Preservation of ram semen has an impact on the exposure artificial conditions to spermatozoa which was followed by overproduction reactive oxygen species (ROS) and resulted in functional damages spermatozoa. This study aimed determine effect combination chicken, quail, duck egg yolks with green tea extract (GTE) quality Sapudi at 5°C storage. Ejaculates more than 70% motility were divided into four groups. Semen extended 1 : 10 citrated-chicken yolk extender (control group, T0), citrated chicken (T1), quail (T2), (T3) added 0.05 mg GTE per 100 mL extender. Extended kept a tube, put beaker containing distilled water stored refrigerator (5°C). Spermatozoa motility, viability, plasma membrane integrity (PMI) evaluated daily for five days. The results showed that citrate could maintain highest PMI days (p <0.05). It be concluded addition (Camellia sinensis) beneficial maintaining chilled temperatures. Further is needed use freezing semen.